"The HIV‐1 packaging system is the most widely used pseudovirus packaging system. To make this packaging system, HIV genes are selectively cloned into DNA vectors. Specifically, 2 to 4 plasmids are used as the vectors, a strategy that aims to minimize viral gene recombination and thereby reduce the possibility of reversion to the WT virus. Table 1 lists the currently used HIV‐1‐based systems. "
This is for cell-based assays, not for "gain of function experiments." I'm not going to explain cell-based assays to you. Instead of jumping to conclusions, you should have researched terms you don't understand.
I explained exactly how this happened and gave a very plausible mechanism behind it. If you don't believe me, that's fine. Say, how long have you worked in vaccine research?
You are misinterpreting what the article is saying. They used a pseudovirus system of HIV origin.
https://onlinelibrary.wiley.com/doi/full/10.1002/rmv.1963
"The HIV‐1 packaging system is the most widely used pseudovirus packaging system. To make this packaging system, HIV genes are selectively cloned into DNA vectors. Specifically, 2 to 4 plasmids are used as the vectors, a strategy that aims to minimize viral gene recombination and thereby reduce the possibility of reversion to the WT virus. Table 1 lists the currently used HIV‐1‐based systems. "
This is for cell-based assays, not for "gain of function experiments." I'm not going to explain cell-based assays to you. Instead of jumping to conclusions, you should have researched terms you don't understand.
I explained exactly how this happened and gave a very plausible mechanism behind it. If you don't believe me, that's fine. Say, how long have you worked in vaccine research?